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目的 研究SP、CGRP及VIP在心脏表面 3个主要神经节丛 ,右心房神经节丛 (RAGP)、心房背侧神经节丛 (DAGP)及主动脉与肺动脉间神经节丛 (A PGP)的化学性质和定位分布。方法 应用免疫组织化学ABC法 ,对豚鼠心脏表面 3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。结果 在上述三个神经节丛内 ,未发现SP及CGRP免疫反应阳性神经元 ,但可见少量SP免疫反应阳性神经纤维分布 ,CGRP免疫反应阳性神经纤维数量较SP阳性纤维密集 ,尤其在A PGP更为明显 ;VIP免疫反应阳性神经元和神经纤维分布最为密集 ,交织成神经节丛。结论 豚鼠心脏表面主要神经节丛内确实存在SP、CGRP及VIP ,并且这 3种神经肽可能直接参与心肌细胞和心脏血管活动的调控。
Objective To investigate the effects of SP, CGRP and VIP on the surface of the heart, including three major ganglion plexus, right atrial ganglion (RAGP), atrial dorsal plexus (DAGP) and aortic and pulmonary plexus ganglion Nature and location distribution. Methods Immunohistochemical ABC method was used to detect the neurons and nerve fibers of the three major ganglion plexus on the surface of guinea pig heart by immunohistochemical staining and light microscopy. Results SP and CGRP immunoreactive neurons were not found in the three ganglion plexus, but a small number of SP immunoreactive positive nerve fibers were observed. The number of CGRP immunoreactive nerve fibers was more dense than SP positive fibers, especially in A PGP Is obvious; VIP immunoreactive positive neurons and nerve fibers are most densely distributed, interwoven into ganglion plexus. Conclusion SP, CGRP and VIP do exist in the major ganglion plexus on the surface of guinea pig heart, and these three neuropeptides may be directly involved in the regulation of myocardial cells and cardiovascular activity.