以球子蕨成熟孢子为外植体,研究了不同激素及浓度对其孢子萌发、愈伤组织诱导、丛生芽分化及生根的影响。结果表明:孢子萌发最适培养基为1/2MS+2%蔗糖,20d后萌发率达55.7%;诱导愈伤组织的最适培养基为MS+0.5mg·L-1KT+0.5mg·L-12,4-D,诱导率达36%,愈伤组织为绿色颗粒状;颗粒状愈伤组织在不添加激素的MS培养基中即可生长出大量丛生芽,转化率可达49.3%;低浓度(0.2mg·L-1)的IAA可有效促进幼孢子体苗生根。 The mature spores of Brassica juncea were used as explants to study the effects of different hormones and their concentrations on their spore germination, callus induction, shoot differentiation and rooting. The results showed that the optimum culture medium for spore germination was 1 / 2MS + 2% sucrose, and the germination rate reached 55.7% after 20 days. The optimum medium for inducing callus was MS + 0.5mg · L-1KT + 0.5mg · L- 12,4-D, the induction rate of 36%, the callus of the green granular; granular callus in MS medium without hormone to grow a large number of clusters of buds, the conversion rate of up to 49.3%; low IAA at a concentration of 0.2 mg · L -1 could effectively promote the rooting of young sporophytes.