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报道了电流型乙醇生物传感器。该传感器以甲苯胺蓝键合修饰浸蜡石墨电极为基 体电极，将醇脱氢酶、烟酰胺腺嘌呤二核苷酸（ＮＡＤ＋）同时固定在蚕丝蛋白膜上，成为无试剂 的醇传感器。在 ｐＨ ８． ５的 Ｔｒｉｓ－ＨＣｌ介质中，该传感器的响应电流与乙醇浓度在 ５． ０× １０－５～ １．１０×１０－３ｍｏｌ／Ｌ范围内有良好的线性关系。响应时间为２０ｓ。本文讨论了影响传感器响应 的各种因素。用该传感器测定了啤酒中乙醇的含量，结果与气相色谱法一致。
Reported current type ethanol biosensors. The sensor uses toluidine blue-bonded modified dipolar graphite electrode as the base electrode, and alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD +) are simultaneously immobilized on the silk fibroin membrane to become a reagentless alcohol sensor. At pH 8. 5 Tris-HCl medium, the sensor response current and ethanol concentration of 5. 0 × 10-5 ~ 1.10 × 10-3mol / L within a good linear relationship. Response time is 20s. This article discusses the various factors that affect the sensor’s response. The sensor was used to determine the content of ethanol in beer. The result was consistent with that of gas chromatography.