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以链脲佐菌素诱导的糖尿病小鼠为实验模型,考察蛹虫草子实体粗多糖(BP)、菌丝体粗多糖(MP)以及胞外粗多糖(EP)的降血糖活性。结果显示只有EP具有显著的降血糖活性。进一步分别对糖尿病组、EP组的小鼠,以刀豆蛋白A刺激脾脏细胞检测其IL-4和IFN-γ水平,以脂多糖刺激脾脏巨噬细胞检测其IL-6和TNF-α水平。结果表明:与糖尿病组相比,EP组IL-4、IFN-γ水平及IFN-γ/IL-4均显著降低,而IL-6水平显著增加,从而导致TNF-α/IL-6比值降低,该结果与EP体外实验结果相符。因此,EP降血糖的免疫学机制可能在于通过提高脾脏巨噬细胞IL-6的水平诱导Th细胞向Th2细胞的分化,从而抑制糖尿病的发病进程。
Diabetic mice induced by streptozotocin were used as experimental models to investigate the hypoglycemic activity of crude polysaccharides (BP), mycelium crude polysaccharides (MP) and extracellular polysaccharides (EP) of Cordyceps militaris. The results showed that only EP had significant hypoglycemic activity. Furthermore, the levels of IL-4 and IFN-γ in spleen cells of ConA and EP groups were detected by Concanavalin A, and the levels of IL-6 and TNF-α in spleen macrophages were detected by lipopolysaccharide stimulation. The results showed that compared with diabetic group, the levels of IL-4, IFN-γ and IFN-γ / IL-4 in EP group were significantly decreased, while the levels of IL-6 were significantly increased, leading to the decrease of TNF-α / IL- , The result is consistent with EP in vitro experiment results. Therefore, EP hypoglycemic immunological mechanism may lie in the level of the spleen macrophage IL-6 induced Th cells to Th2 cells differentiation, thereby inhibiting the pathogenesis of diabetes.