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目的探讨B族链球菌荚膜多糖-破伤风类毒素结合物(GBS-CPS-TT),经不同免疫途径免疫小鼠的可行性及免疫剂量。方法经滴鼻、灌胃、皮下注射途径免疫小鼠,各组均免疫3次,每次免疫间隔时间2周,免疫后1周时采集血清,采用间接酶联免疫吸附实验(ELISA)检测血清中特异性IgG的抗体滴度。应用统计软件SPSS13.0对数据进行统计学分析,组间均数比较采用方差分析。结果 GBS-CPS-TT经滴鼻免疫、灌胃免疫和皮下注射均可以诱导产生特异性血清IgG抗体。IgG抗体滴度分别为:滴鼻5μg组(2.671±0.207);滴鼻10μg组(2.911±0.263);皮下注射5μg组(2.866±0.251);灌胃10μg组(2.851±0.224)。滴鼻10μg组诱导产生的IgG抗体滴度明显高于滴鼻5μg组(P<0.01)。结论 GBS-CPS-TT经滴鼻免疫小鼠后可产生系统性免疫应答。
Objective To investigate the feasibility and immunization dose of Group B streptococcal capsular polysaccharide - tetanus toxoid conjugate (GBS-CPS-TT) and immunization of mice by different immunization routes. Methods The mice were immunized intranasally, intragastrically and subcutaneously. The mice in each group were immunized three times for 2 weeks at each immunization interval and one week after immunization. Serum was collected by indirect enzyme - linked immunosorbent assay (ELISA) Antibody titers of medium specific IgG. The statistical software SPSS13.0 was used for statistical analysis of the data, the analysis of variance was used to compare the mean between groups. Results GBS-CPS-TT was immunized with intranasal, gavage and subcutaneous injection can induce specific serum IgG antibody. IgG antibody titers were: intranasal 5μg group (2.671 ± 0.207), intranasal 10μg group (2.911 ± 0.263), subcutaneous injection of 5μg group (2.866 ± 0.251) and intragastric administration of 10μg group (2.851 ± 0.224). The titer of IgG antibody induced by intranasal 10μg group was significantly higher than that of intranasal 5μg group (P <0.01). Conclusion GBS-CPS-TT mice can be immunized intranasally to produce systemic immune response.