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将乙肝病毒（ＨＢＶ）的反义或正义寡核苷酸，加入能复制ＨＢＶ的肝癌细胞模型ＨｅｐＧ２．２．１５中，通过检测其上清中ＨＢｓＡｇ和ＨＢｅＡｇ的含量，初步分析了反义寡核苷酸潜在的抗ＨＢＶ能力。针对Ｃ基因区１９７４─１９５５的反义链（ＣＳＰ）在１５μｍｏｌ／Ｌ浓度下可抑制８４．８％的ＨＢｓＡｇ表达和４０．０％的ＨＢｅＡｇ表达，停药２日时抑制率仍分别为９３．５％和３９．０％。实验期间未发现反义链对上述细胞形态或细胞生长状况有明显影响。结果提示反义寡核苷酸ＣＳＰ在实验条件下具有一定的抑制细胞ＨＢＶ表达的能力。
Antisense or sense oligonucleotide of Hepatitis B virus (HBV) was added into HepG2.2.15 hepatoma cell model which can replicate HBV. By detecting the content of HBsAg and HBeAg in the supernatant, the antisense oligonucleotides Potentials have potential anti-HBV ability. The antisense strand (CSP) targeting C gene region 1974- 1955 inhibited 84.8% of HBsAg expression and 40.0% of HBeAg expression at a concentration of 15μmol / L, and the inhibition rates were still 93% on the 2nd day. 5% and 39.0%. During the experiment, no antisense strand was found to have a significant effect on the above cell morphology or cell growth status. The results suggest that antisense oligonucleotide CSP under the experimental conditions have a certain ability to inhibit cellular HBV expression.