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目的观察海马、边缘区及相关脑区的基因表达谱是否存在差异,期望寻找到海马、边缘区的特异基因。方法从引产胎儿胎脑中准确取出海马、杏仁核、边缘区和尾壳核。用mRNA差异显示技术分析上述脑区的基因表达差异,并对海马的一个差异片段进行了克隆、测序。结果4个脑区相比,海马、边缘区各发现2个差异片段,海马的一个差异片段长度为277 bp,序列与编号为AC0049人类克隆序列完全同源。结论海马、边缘区等不同脑区间基因表达存在差异。
Objective To observe whether there are differences in gene expression profiles in hippocampus, marginal zone and related brain regions, and to find specific genes in hippocampus and marginal zone. Methods The hippocampus, amygdala, marginal zone and caudate putamen were accurately removed from fetal brain of induced labor. The differences in gene expression in the brain regions were analyzed by mRNA differential display technique. A differential fragment of the hippocampus was cloned and sequenced. Results There were two different fragments in the hippocampus and the marginal zone of the four brain regions. One difference fragment in the hippocampus was 277 bp in length. The sequence was completely homologous to the human cloned sequence of AC0049. Conclusion There are differences in gene expression in different brain regions such as hippocampus and marginal zone.