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目的:通过盆栽试验研究施加外源硅对盐胁迫下甘草叶片显微结构的影响。方法:设置2个盐水平,分别为12 g NaCl/kg干土、15 g NaCl/kg干土,同时设置对照(CK)。以硅酸钾(K_2SiO_3-nH_2O化学纯)作为硅源,选择给15 g/kg盐处理添加硅0.6 g/kg,利用光学显微镜、石蜡切片技术进行观察与分析。结果:不同浓度NaCl处理下的甘草叶片解剖结构与对照相比发生了显著变化,表现为叶肉细胞数目减少、细胞形状改变、排列松散,部分细胞破裂,施加外源硅后靠近栅栏组织处的海绵细胞转变为栅栏组织,叶肉细胞排列紧密且细胞结构完整,维管束面积增加,木质部导管数目有所增多。结论:表明盐胁迫对甘草叶片细胞显微结构具有损伤作用,施加外源硅可在一定程度上缓解盐胁迫对甘草叶肉细胞、维管束结构的损害,维持正常的细胞形态。
Objective: Pot experiments were conducted to study the effects of exogenous silicon on the microstructure of licorice leaves under salt stress. Methods: Two salt levels were set up, 12 g NaCl / kg dry soil and 15 g NaCl / kg dry soil, respectively. Meanwhile, CK was set. With potassium silicate (K 2 SiO 3 -nH 2 O chemical pure) as the silicon source, silicon (0.6 g / kg) was added at 15 g / kg salt treatment. The observation and analysis were made by optical microscope and paraffin section. Results: The anatomical structures of Glycyrrhiza uralensis Fisch under different concentrations of NaCl changed significantly compared with the control. The results showed that the number of mesophyll cells decreased, cell shape changed, loosely arranged and some cells ruptured. Sponges near the palisade tissue after applying exogenous silicon Cells transformed into palisade tissue, mesophyll cells arranged closely and cell structure integrity, vascular bundle area increased, the number of xylem catheters increased. CONCLUSION: Salt stress can damage the microstructure of Glycyrrhiza glabra leaf cells. Exogenous application of Si can alleviate the damage of Glycyrrhiza urophylla to some extent and maintain the normal cell morphology to a certain extent.