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本世纪五十年代末,人们开始注意到铬的生理活性。铬以三价铬的活性化合物的形式协同胰岛素在体内外的作用,已被实验所证实。,由于铬在人体内含量很低,准确测定其含量比较困难,使铬的测定成为研究其生理作用的一大制约因素。本文采用角平台石墨管为原子化器,建立了无火焰原子吸收光谱法测定血浆铬的方法。 1 试验部分1.1 仪器与主要试剂 WFX—1F_2型原子吸收分光光度计(北京第二光学仪器厂) 全热解角平台石墨管(核工业部五所) 四次蒸馏水(电导率1×10~(-6)s·cm~(-1)) 硝酸(半导体纯,MOS) 铬标准贮备液:1000μg·ml~(-1) 所用器皿均经硝酸(1+1)浸泡48h以上,重蒸水冲洗,四次蒸馏水浸泡12h,干燥备用。
The late 1950s, people began to notice the physiological activity of chromium. The role of chromium in the form of an active compound of trivalent chromium in conjunction with insulin in vitro and in vivo has been experimentally demonstrated. Due to the low content of chromium in the human body, it is difficult to accurately determine the content of chromium, making the determination of chromium a major factor to study its physiological role. In this paper, the angular platform graphite tube as a atomizer, established by flame atomic absorption spectrometry method for the determination of plasma chromium. 1 Experimental 1.1 Instruments and Major Reagents WFX-1F 2 Atomic Absorption Spectrophotometer (Beijing No.2 Optical Instrument Factory) Total Pyrolysis Angle Platform Graphite Tube (Five Ministry of Nuclear Industry) Four Distilled Water (Conductivity 1 × 10 ~ (- 6) s · cm -1) Standard stock solution of nitric acid (semiconductor pure, MOS) chrome: 1000μg · ml -1 All the vessels used were soaked in nitric acid (1 + 1) for more than 48h, rinsed with distilled water, Four distilled water soak 12h, dry spare.