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烟草中已开发的SSR 标记只有一部分定位到遗传图谱中(记为M-SSR),而大部分还没有定位(记为 N-SSR).本研究利用已发表的5 000 多对烟草SSR 标记引物和包含2 000 多个SSR 标记的高密度遗传图谱以及3 个烟草品种基因组序列的数据,通过e-PCR 将SSR 标记定位到烟草基因组中,并根据位于相同支架的M-SSR 和N-SSR 标记的连锁关系,将92 个N-SSR 标记定位到烟草遗传图中.本研究结果增加了烟草 SSR 遗传图的密度,证明了这种电子遗传定位方法的可行性,可在不同物种中应用.“,”Among t he SSR markers developed in tobacco,only part of them have been genetically mapped (denoted as M-SSR),while most are still unmapped (denoted as N-SSR).In this study,using the published data of over 5 000 tobacco SSR marker primers and a high-density genetic map of tobacco containing more 2 000 SSR markers as well as the genomic sequences of three tobacco varieties,the SSR markers were mapped to the tobacco genome by e-PCR,and then 92 N-SSR markers were mapped onto the genetic map according to the linkage relationship between M-SSR and N-SSR markers located in the same scaffolds.The results of this study increased the density of the tobacco SSR genetic map,demonstrating the feasibility of this in silico genetic mapping method,which is applicable to different species.