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目的:研究白花丹参根制剂对体外培养的人胃癌细胞BGC823增殖和凋亡的影响．方法:用不同浓度白花丹参根制剂处理胃癌细胞BGC823后,噻唑蓝(MTT)检测细胞的相对存活率,流式细胞仪Annexin V／PI双染法检测BGC823细胞凋亡,激光扫描共聚焦显微镜检测细胞形态．对结果进行统计分析,均数比较用t检验．结果:不同浓度白花丹参根制剂作用于BGC823细胞后,细胞的存活率明显下降且呈剂量依赖性(0．4 g／L:91．7％±10．6％;0．8 g／L:66．8％±5．1％;1 g／L:57．5％±9．6％;1．5 g／L:32．6％±7.3％;2 g／L:29．4％±9．4％)．镜下可见凋亡细胞典型形态,流式细胞术分析表明经过白花丹参根制剂处理后早期凋亡细胞数和晚期凋亡细胞数与对照组相比均显著增加(2．950％±1．575％vs4．105％±2．393％．P<0．05;3．848％±2．264％vs 21．465％±6．474％,P<0．05)．结论:白花丹参根制剂能有效地抑制胃癌细胞增生和诱导细胞凋亡．
Objective: To study the effects of Salvia miltiorrhiza root on proliferation and apoptosis of human gastric cancer cell line BGC823 in vitro. Methods: The relative survival rates of BGC823 cells treated with different concentrations of Salvia miltiorrhiza root preparations were determined by MTT assay. The apoptosis of BGC823 cells was detected by flow cytometry Annexin V / PI double staining. Cell morphology. The results of statistical analysis, compared with the t-test. Results: After treated with different concentrations of Salvia miltiorrhiza root, the survival rate of BGC823 cells was significantly decreased and dose dependent (0.4 g / L: 91.7% ± 10.6%; 0.8 g / L: 66.8% ± 5.1%, 1 g / L: 57.5% ± 9.6%, 1.5 g / L: 32.6% ± 7.3%, 2 g / L: 29.4% ± 9.4%). The morphological changes of apoptotic cells were observed by flow cytometry. The number of apoptotic cells and the number of late apoptotic cells were significantly increased after treated with Salvia miltiorrhiza root (2.950% ± 1.575 % Vs 4.105% ± 2.393%. P <0.05; 3.848% ± 2.264% vs 21.465% ± 6.474%, P <0.05). Conclusion: Salvia miltiorrhiza root preparations can effectively inhibit gastric cancer cell proliferation and induce apoptosis.