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目的探讨并改进盐酸副玫瑰苯胺比色法测定亚硫酸盐的方法。方法对于水不溶性的固体样品,可以用超声法(40min)或振荡法(5min)来代替浸泡法进行样品前处理。配制0.02%的盐酸副玫瑰苯胺使用溶液时,可吸取0.1%盐酸副玫瑰苯胺溶液20ml后直接加入一定量的(1+1)盐酸使盐酸的浓度为0.20～1.00mol/L之间使用。结果浸泡5h与超声40min2种样品处理方法测定的结果经t检验差异无统计学意义(P>0.05);同时浸泡5h与振荡50min2种方法测定的结果差异也无统计学意义(P>0.05)差异。盐酸副玫瑰苯胺溶液中的盐酸介质的浓度在0.70～1.00mol/L之间,空白吸光度值较低(0.062～0.043)并且灵敏度较高。结论改进后方法处理样品省时,实验中盐酸酸度范围的确定及标准曲线线性范围扩大,均给操作者带来方便。
Objective To explore and improve the method of pararosal hydrochloride colorimetric determination of sulfite. Methods For water-insoluble solid samples, the sample can be pre-treated by sonication (40 min) or shaking (5 min) instead of by immersion. Preparation of 0.02% pararosanide hydrochloride solution used, can absorb 0.1% pararosaniline hydrochloride solution 20ml directly after a certain amount of (1 + 1) hydrochloric acid hydrochloric acid concentration of 0.20 ~ 1.00mol / L use between. Results There was no significant difference between the two methods of soaking (5h) and ultrasound (40min) in the results of t test (P> 0.05) . Hydrochloric acid pararosaniline solution of hydrochloric acid medium concentration between 0.70 ~ 1.00mol / L, the blank absorbance value is low (0.062 ~ 0.043) and high sensitivity. Conclusion The improved method to deal with the sample province, the determination of hydrochloric acid acidity range of the experiment and the linear expansion of the standard curve, are convenient for the operator.