论文部分内容阅读
目的探讨自身免疫性肝炎(AIH)患者外周血T淋巴细胞表达活化指标HLA-DR、CD25、CD38在AIH发生发展中的机制及临床检测价值。方法应用流式细胞术检测73例AIH组发病期患者、61例AIH组缓解期患者和35例健康对照组外周血T淋巴细胞免疫活化指标(HLA-DR、CD25、CD38),对AIH组发病期、AIH组缓解期与健康对照组的免疫活化指标表达百分率进行比较。结果 AIH组发病期HLA-DR~+、CD3~+HLA-DR~+、CD8~+HLA-DR~+、CD25~+、CD38~+、CD8~+CD38~+表达百分率均高于健康对照组;各组间百分率均数的比较,差异均有统计学意义(P<0.05)。AIH组缓解期HLA-DR~+、CD3~+HLA-DR~+、CD8~+HLA-DR~+、CD25~+、CD38~+、CD8~+CD38~+表达百分率低于发病期,差异均有统计学意义(P<0.05)。结论 T淋巴细胞活化指标HLA-DR、CD25、CD38在AIH发病期表达增加,缓解期表达降低;CD4~+CD25~+在AIH发病期表达降低,缓解期表达增加。
Objective To investigate the mechanism and clinical value of the expression of HLA-DR, CD25 and CD38 in peripheral blood T lymphocytes of patients with autoimmune hepatitis (AIH). Methods The immune activation indexes (HLA-DR, CD25, CD38) in peripheral blood of 73 AIH patients, 61 AIH remission patients and 35 healthy controls were detected by flow cytometry. The AIH group remission and healthy control group immunocompromised indicators were compared. Results The percentage of HLA-DR +, CD3 + HLA-DR +, CD8 + HLA-DR +, CD25 +, CD38 +, CD8 + CD38 + in AIH group was higher than that in healthy controls Group; the percentages between groups were compared, the differences were statistically significant (P <0.05). The percentage of HLA-DR +, CD3 + HLA-DR +, CD8 + HLA-DR +, CD25 +, CD38 +, CD8 + CD38 + in AIH group was lower than that in AIH group All were statistically significant (P <0.05). Conclusion The expressions of HLA-DR, CD25 and CD38 in T lymphocytes were increased in AIH and decreased in remission stage. The expression of CD4 ~ + CD25 ~ + was decreased in AIH and increased in remission stage.