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目的研究黑紫獐牙菜Swertia atroviolacea叶片的化学成分。方法采用硅胶柱色谱、Zorbax Prep HT GF反相柱色谱及Sephadex LH-20凝胶柱色谱等现代分离技术进行分离纯化,根据理化性质和波谱数据鉴定化合物结构,采用MTT法测定化合物1对5株人体癌细胞株NB4、A549、SHSY5Y、PC3和MCF-7的体外细胞毒活性。结果从黑紫獐牙菜叶片95%乙醇提取物中分离得到5个化合物,分别鉴定为3-(3-甲基-2-氧代-丁-3-烯基)-6-乙酰基-1,5-二甲氧基酮(1)、1,2,3,4-四氢-1,4,6,8-四羟基酮(2)、1,3-二甲氧基-8-羟基酮(3)、1,2,6,8-四羟基酮(4)、熊果酸(5)。化合物1对NB4、A549、SHSY5Y、PC3、MCF-7细胞的IC50值均小于10μmol/L,其中对A549和MCF-7细胞具有较高的细胞毒活性,其IC50值分别为5.2和3.8μmol/L。结论化合物1为新化合物,命名为黑紫獐牙菜素A,此化合物具有显著的细胞毒活性。
Objective To study the chemical constituents of Swertia atroviolacea leaves. Methods The compounds were isolated and purified by silica gel column chromatography, Zorbax Prep HT GF reversed-phase column chromatography and Sephadex LH-20 column chromatography. The structures of the compounds were identified by their physico-chemical properties and spectral data. In vitro cytotoxic activity of human cancer cell lines NB4, A549, SHSY5Y, PC3 and MCF-7. Results Five compounds were isolated from 95% ethanolic extracts of A. asteraceae and identified as 3- (3-methyl-2-oxo-but-3-enyl) (1), 1,2,3,4-tetrahydro-1,4,6,8-tetrahydroxycholorone (2), 1,3-dimethoxy-8 - Hydroxychloreone (3), 1,2,6,8-Tetrahydroxycholicone (4), Ursolic acid (5). The IC50 values of compound 1 against NB4, A549, SHSY5Y, PC3 and MCF-7 cells were all less than 10μmol / L, with high cytotoxicity against A549 and MCF-7 cells, with IC50 values of 5.2 and 3.8μmol / L. Conclusion Compound 1 is a new compound, named black violet dewprin A, which has significant cytotoxic activity.