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AIM: To develop a specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the phar-macokinetic study of magnesium lithospermate B (MLB), and study the pharmacokinetics of MLB after iv administration in beagle dogs. METHODS: Each beagle dog was iv administered MLB 3, 6, and 12 mg/kg random. The serum drug concentration was determined by specific liquid chromatography-tandem mass spectrometry (LC-MS/ MS) assays. The pharmacokinetic parameters were calculated by Drug and Statistics version 1.0 program. RESULTS: The calibration curve for MLB was linear over a range of 16-4096μg/L with coefficients of correlation >0.999. The intra- and inter-day precisions (CV) of analysis were <10 %, and accuracy ranged from 90 % to 113 %. After iv administration of MLB at the doses of 3, 6, and 12 mg/kg, the C0 values for MLB were estimated to be of 24, 47, and 107 mg/L, respectively. The AUC increased with the increasing doses for iv administration, and the mean AUC0-t values were 109.3, 247.9, and 5
AIM: To develop a specific liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the phar-macokinetic study of magnesium lithospermate B (MLB), and study the pharmacokinetics of MLB after iv administration in beagle dogs. METHODS: Each The serum drug concentration was determined by specific liquid chromatography-tandem mass spectrometry (LC-MS / MS) assays. The pharmacokinetic parameters were calculated by Drug and Statistics version 1.0 program. RESULTS: The calibration curve for MLB was linear over a range of 16-4096 μg / L with coefficients of correlation> 0.999. The intra- and inter-day precisions (CV) of analysis were <10%, and accuracy ranged from 90% to 113%. After iv administration of MLB at the doses of 3, 6, and 12 mg / kg, the C0 values for MLB were estimated to be 24, 47, and 107 mg / L, respectively. with the increasing doses for iv administration, and the mean AUC0-t values were 109.3, 247.9, and 5