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目的 :研究大鼠急性心肌梗死后心肌细胞凋亡和半胱天冬蛋白酶 3 (Caspase 3 )的表达及卡维地洛的防治作用。 方法 :冠状动脉结扎术后 2 4h存活的雌性SD大鼠 83只随机分为心肌梗死对照组 (MI组 ,n =43 )和卡维地洛[10mg/(kg·d) ]治疗组 (C组 ,n =40 )两组 ,另设假手术组 (S组 ,n =2 7) ;最终血流动力学资料完整的上述各组大鼠数分别为 11、 13、 9、 15、 10和 10只。C组于术后 2 4h直接灌胃法给药。末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法 (TUNEL)和脱氧核糖核酸 (DNA)凝胶电泳检测心肌细胞凋亡。免疫组化方法和Western印迹法检测Cas pase 3的表达。 结果 :与S组相比 ,MI组的左心室收缩压 (LVSP)和左心室内压最大上升和下降速率 (±dp/dt)均显著降低 (P <0 0 5～ 0 0 1) ,左心室舒张末压 (LVEDP)均显著升高 (P <0 0 5～ 0 0 1) ,且 4W时升高更为显著 (P <0 0 1) ;梗死区或疤痕区、梗死边缘区和非梗死区的心肌细胞凋亡指数均显著升高 (P <0 0 5～ 0 0 1) ,Caspase 3表达也均显著增高 (P <0 0 5～ 0 0 1)。与MI组相比 ,C组 48h的LVSP、±dp/dt和心率均显著降低 (P <0 0 5～ 0 0 1) ,但LVEDP及上述各部位的心肌细胞凋亡指数和Caspase 3的表达均无显著变化 ;而C组 4W的
AIM: To investigate the apoptosis of cardiomyocytes and the expression of Caspase 3 and the preventive and therapeutic effects of carvedilol in rats after acute myocardial infarction. Methods: Eighty-three female Sprague-Dawley rats survived 24 hours after coronary artery occlusion were randomly divided into three groups: MI group (n = 43) and carvedilol (10 mg / (kg · d) Group, n = 40), sham operation group (group S, n = 27). The number of the rats in the final hemodynamic data was 11, 13, 9, 15, 10 and 10 only Group C was administrated directly 24 h after operation. Cardiomyocyte apoptosis was detected by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL) and DNA gel electrophoresis. Immunohistochemistry and Western blotting were used to detect Caspase 3 expression. RESULTS: Compared with S group, LVSP and ± dp / dt of left ventricular pressure were significantly decreased in MI group (P <0.05 ~ 0.01), left (LVEDP) increased significantly (P <0.05 ~ 0.01), and increased significantly at 4W (P <0.01). The infarct area or scar area, infarct border area and non-infarct area The apoptotic index of myocardial cells in infarction area were significantly increased (P <0.05-0.01) and Caspase 3 expression was also significantly increased (P <0.05-0.01). Compared with MI group, the LVSP, ± dp / dt and heart rate of group C were significantly decreased at 48h (P <0.05-0.01). However, the apoptosis index and the expression of Caspase 3 No significant change; while C group 4W