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目的探讨不同程度肺挫伤患者外周血白细胞可溶性髓样细胞触发受体-1(soluble triggering receptor expressed on myeloid cell-1,s TREM-1)mRNA的动态表达变化及其临床意义。方法 30例健康体检者(A组)为对照组。用反转录聚合酶链反应(RT-PCR)检测不同程度肺挫伤患者外周血白细胞s TREM-1 mRNA的表达水平,包括轻度肺挫伤(B组,20例)、中度肺挫伤(C组,16例),重度肺挫伤(D组,9例),并检测患者入院时及入院后第1、3、7天s TREM-1 mRNA表达的动态变化。结果在同一时点,B→C→D组s TREM-1mRNA表达逐渐增高,差异有统计学意义(P均<0.01),即随肺挫伤的加重s TREM-1mRNA表达水平增高。同一组中,从入院时到入院第3天,s TREM-1mRNA表达水平逐渐增高,差异有统计学意义(P均<0.05);至入院第7天其表达水平下降至入院时水平,但中、重度肺挫伤仍高于对照组(A组),差异有统计学意义(P<0.05或P<0.01)。结论外周血白细胞s TREM-1的表达变化与肺挫伤严重程度密切相关,并随着病情好转而降低。
Objective To investigate the dynamic expression of soluble TRAIL-1 (TRAIL-1) mRNA in peripheral blood leukocytes and its clinical significance in patients with pulmonary contusion. Methods 30 healthy subjects (group A) as control group. The levels of s-TREM-1 mRNA in peripheral leukocytes in patients with pulmonary contusion were detected by reverse transcription polymerase chain reaction (RT-PCR), including mild pulmonary contusion (group B, 20 cases), moderate pulmonary contusion (N = 16) and severe pulmonary contusion (n = 9). The changes of s TREM-1 mRNA expression on the 1st, 3rd, 7th day after admission were detected. Results At the same time point, the expression of TREM-1 mRNA in B → C → D group increased gradually (P <0.01), which means the expression of s TREM-1 mRNA increased with the increase of pulmonary contusion. In the same group, the expression of s-TREM-1 mRNA gradually increased from the time of admission to the third day of hospitalization, and the difference was statistically significant (P <0.05). The expression level of TREM-1 decreased to the level of admission on the 7th day of admission, , Severe pulmonary contusion was still higher than the control group (A group), the difference was statistically significant (P <0.05 or P <0.01). Conclusion The expression of s-TREM-1 in peripheral blood leucocytes is closely related to the severity of pulmonary contusion and decreases as the condition improves.