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目的:探讨丹参在重症急性胰腺炎(SAP)大鼠结肠壁神经丛ACH、VIP、NOS表达与胃肠动力关系。方法:50只SD大鼠,随机分为假手术组(AF组)、丹参处理假手术组(SI-AF组)、重症急性胰腺炎组(SAP组)、丹参处理重症急性胰腺炎组(SI-SAP组)以及空白组,每组各10只。建立重症急性胰腺炎大鼠模型;干预结束后比较结肠壁神经丛乙酰胆碱(ACH)、血管活性肠肽(VIP)以及一氧化氮合成酶(NOS)的表达与胃肠动力关系。结果:干预后与SAP组比较,SI-SAP组胰腺组织病理学评分较低(P<0.05),结肠肌间丛以及结肠黏膜下丛ACH表达较高(P<0.05),NOS表达较低(P<0.05),VIP表达较低(P<0.05),IMC周期以及Ⅰ、Ⅱ相时间较低(P>0.05);Ⅲ相时间、Ⅲ相峰电振幅及频率较高(P>0.05)。结论:丹参在重症急性胰腺炎大鼠结肠壁神经丛的治疗中有显著作用,其治疗机制与显著提高结肠壁神经丛ACH表达,下调NOS表达以及VIP表达水平,改善胃肠动力作用有关,缓解胰腺组织损伤。
AIM: To investigate the relationship between the expression of ACH, VIP and NOS in colonic plexus and gastrointestinal motility of Salvia miltiorrhiza in rats with severe acute pancreatitis (SAP). Methods: Fifty Sprague-Dawley rats were randomly divided into four groups: sham operation group (AF group), Salvia miltiorrhiza sham operation group (SI-AF group), severe acute pancreatitis group (SAP group) -SAP group) and blank group, each group of 10. The rat model of severe acute pancreatitis was established. After the intervention, the relationship between the expression of nerve plexus acetylcholine (ACH), vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) and gastrointestinal motility was compared. Results: Compared with SAP group, the histopathological score of SI in SAP group was lower (P <0.05), the expression of ACH in colonic myenteric plexus and colonic mucosa was higher (P <0.05) and NOS was lower (P < (P <0.05), VIP expression was lower (P <0.05), IMC cycle and phase Ⅰ, Ⅱ phase time was lower (P> 0.05); Ⅲ phase time, phase Ⅲ peak amplitude and frequency of higher (P> 0.05). Conclusion: Salvia miltiorrhiza has a significant role in the treatment of colon wall plexus in rats with severe acute pancreatitis. Its therapeutic mechanism is related to the significant increase of ACH expression in colonic plexus, down-regulation of NOS expression and VIP expression, improvement of gastrointestinal motility, Pancreatic tissue injury.