为了解粘附分子（Ａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅｓ）在Ｔ细胞激活中的作用及其机制，作者采用ＴＣＲ－ＣＤ３复合物、ＬＦＡ－１、ＬＦＡ－２、ＣＤ４４和ＣＤ４５特异性单克隆抗体刺激，ＦｃγＲＩＩ阳性或阴性肿瘤细胞刺激，以及刺激阻断的方法，以ＩＦＮ－γ定量检测作为Ｔ细胞激活的标志，研究了ＴＣＲ－ＣＤ３复合物交联（Ｃｒｏｓｓ－ｌｉｎｋｉｎｇ），交联的不同形式，ＬＦＡ－１、ＬＦＡ－２、ＣＤ４４和ＣＤ４５分子对Ｔ细胞激活的影响及其机制。结果提示：ＴＣＲ－ＣＤ３复合物的持久性交联是Ｔ细胞体外激活的必要条件，上述４种粘附分子是Ｔ细胞激活的共刺激分子，其作用机制不仅是细胞间的粘附，而且还作为各自独立的刺激分子影响Ｔ细胞的信号传递。 To understand the role of adhesion molecules in T cell activation and its mechanism, the authors used TCR-CD3 complexes, LFA-1, LFA-2, CD44 and CD45-specific monoclonal antibodies to stimulate FcγRII-positive or negative tumors (TCR-CD3), cross-linking of TCR-CD3 complex, different forms of cross-linking, LFA-1, LFA- 2, CD44 and CD45 molecules on T cell activation and its mechanism. The results suggest that persistent cross-linking of TCR-CD3 complex is a necessary condition for activation of T cells in vitro. The four adhesion molecules are costimulatory molecules activated by T cells. Their mechanism of action is not only the intercellular adhesion but also Individual stimuli affect T cell signaling.