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目的:研究甘草甜素对大鼠体内苦参碱药动学的影响。方法:采用高效液相色谱法测定大鼠血浆中苦参碱的含量,色谱柱为Diamonsil C1(8250mm×4.6mm,5μm),流动相为乙腈-0.1%H3PO(4三乙胺调pH值至8;35∶65),流速为1mL·min-1,柱温为30℃,检测波长为220nm。实验分为2组,A组灌胃苦参碱(70mg·kg-1),B组灌胃苦参碱(70mg·kg-1)和甘草甜素(70mg·kg-1)。应用3p97软件处理苦参碱的血药浓度-时间数据。结果:A组Cmax=(6.861±0.635)mg·L-1,AUC0～t=(47.105±7.062)mg·h·L-1,tmax=(1.471±0.438)h;B组Cmax=(4.122±0.965)mg·L-1,AUC0～t=(35.507±5.024)mg·h·L-1,tmax=(1.158±0.175)h。经统计学分析,2组之间Cmax、AUC、CL、t1/2α、t1/2β、K21均有显著性差异(P<0.05)。结论:苦参碱与甘草甜素合用,与其单独给药比较,甘草甜素对其在大鼠体内的吸收、分布、代谢及排泄都有不同程度的影响。
Objective: To study the effect of glycyrrhizin on matrine pharmacokinetics in rats. Methods: The content of matrine in rat plasma was determined by HPLC. Diamonsil C1 (8250mm × 4.6mm, 5μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-0.1% H3PO 8; 35:65), the flow rate was 1mL · min-1, the column temperature was 30 ℃ and the detection wavelength was 220nm. The experiment was divided into two groups: group A was treated with matrine (70 mg · kg -1), group B was treated with matrine (70 mg · kg -1) and glycyrrhizin (70 mg · kg -1). Matrine concentration-time data was processed using 3p97 software. Results: Cmax in group A was (6.861 ± 0.635) mg · L-1, AUC0 ~ t was (47.105 ± 7.062) mg · h · L-1 and tmax was (1.471 ± 0.438) 0.965) mg · L-1, AUC0 ~ t = (35.507 ± 5.024) mg · h · L-1, tmax = (1.158 ± 0.175) h. After statistical analysis, Cmax, AUC, CL, t1 / 2α, t1 / 2β, K21 between the two groups were significantly different (P <0.05). Conclusion: Matrine and glycyrrhizin combined with its administration alone, glycyrrhizin on its absorption, distribution, metabolism and excretion in rats have varying degrees of impact.