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采用细胞培养方法 ,以3 H TdR掺入率反映细胞增殖水平 ,研究了白介素 2 (IL 2 )对大鼠源的RC 4B/C垂体瘤细胞系增殖的影响 ,并初步分析了IL 2的作用机制。结果表明 :(1)IL 2 (10～ 10 0 0U/ml)剂量依赖性地显著提高RC 4B/C细胞3 H TdR掺入率。(2 )特异性酪氨酸蛋白激酶 (PTK)抑制剂tyrphostin (1μmol/L)可明显降低RC 4B/C细胞3 H TdR掺入率 ,并可部分阻断IL 2的促细胞增殖作用。(3)特异性蛋白激酶A (PKA)抑制剂H 9(1μmol/L)作用后 ,3 H TdR掺入率明显升高 ;H 9还可加强IL 2的促细胞增殖作用。(4)在本实验中未观察到抗雌激素tamoxifen (1μmol/L)对IL 2促RC 4B/C细胞增殖效应有明显影响。上述结果表明 ,IL 2参与调节垂体瘤细胞的增殖活动 ,并且IL 2的作用与PTK及PKA信号传导途径有关。
The effects of interleukin 2 (IL 2) on the proliferation of RC 4B / C pituitary tumor cell lines were studied by using cell culture method, and the incorporation of 3 H TdR was used to reflect the cell proliferation. The effects of IL 2 mechanism. The results showed that: (1) IL 2 (10 ~ 100 U / ml) significantly increased the incorporation of 3 H TdR in RC 4B / C cells in a dose-dependent manner. (2) tyrphostin (1μmol / L), a specific tyrosine kinase inhibitor (PTK), could significantly reduce the incorporation of 3 H TdR in RC 4B / C cells and partially block the effect of IL 2 on cell proliferation. (3) The incorporation rate of 3 H TdR was significantly increased after the specific inhibitor of protein kinase A (PKA) H 9 (1 μmol / L). H 9 could also promote the proliferation of IL 2. (4) No effect of anti-estrogen tamoxifen (1μmol / L) on the proliferation of IL-2-induced RC 4B / C cells was observed in this experiment. The above results indicate that IL 2 is involved in the regulation of proliferative activity of pituitary tumor cells, and the effect of IL 2 is related to PTK and PKA signaling pathways.