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目的研究肠道微生态在华支睾吸虫致肝损伤中的作用。方法建立华支睾吸虫感染大鼠模型。分别在造模后48 h、18 d和35 d检测血浆内毒素、ALT(谷丙转氨酶)、AST(谷草转氨酶)水平;检测肠黏液中sIgA含量;取肠内容物进行乳酸杆菌、双歧杆菌、肠球菌和肠杆菌的培养及定量分析。结果造模后18 d、35 d,大鼠血浆内毒素、ALT、AST水平明显升高,差异有统计学意义(t_内=8.335、7.612,t_(ALT)=11.815、9.874,t_(AST)=7.433、8.015,P值均<0.01);造模后48 h,肠黏液sIgA水平高于正常组(t=2.752,P<0.05),尤以造模后18 d、35 d升高显著(t=13.118、9.546,P值均<0.01);造模后48 h、18 d和35 d,肠道乳酸杆菌和双歧杆菌数量明显减少(t_乳=2.612、4.142、5.556,t_(双)=2.302、4.565、3.982,P值<0.05或P值<0.01),造模后18 d和35 d,肠杆菌和肠球菌数量明显增多(t_肠=4.562、5.247,t肠=5.366、4.775,P值均<0.01)。结论华支睾吸虫感染可致大鼠肠道菌群发生失调,引发内毒素血症,参与介导对肝细胞的损伤。
Objective To study the role of intestinal microflora in liver injury induced by Clonorchis sinensis. Methods The rat model of Clonorchis sinensis infection was established. Plasma endotoxin, ALT (alanine aminotransferase) and AST (aspartate aminotransferase) levels were measured at 48 h, 18 d and 35 d after the model was established respectively. The content of sIgA in intestinal mucus was detected. The content of intestinal contents was determined by lactobacillus, Bifidobacterium , Enterococcus and Enterobacter culture and quantitative analysis. Results The levels of plasma endotoxin, ALT and AST were significantly increased at 18 d and 35 d after model making in rats (t_ = 8.335, 7.612, t_ (ALT) = 11.815,9.874, t_ (AST ) = 7.433,8.015, P <0.01). The level of sIgA in intestinal mucus was significantly higher than that in the normal group at 48 h after modeling (t = 2.752, P <0.05), especially at 18 and 35 d (t = 13.118, 9.546, P <0.01). The number of Lactobacillus and Bifidobacterium decreased significantly at 48 h, 18 d and 35 d after modeling (t_em = 2.612, 4.142 and 5.556, t_ The number of Enterobacteriaceae and Enterococcus were significantly increased on the 18th day and the 35th day after modeling (t_enter = 4.562,5.247, t = 5.366 , 4.775, P <0.01). Conclusion Clonorchis sinensis infection can cause imbalance of intestinal flora in rats, triggering endotoxemia and participating in the injury of hepatocytes.