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目的了解中华按蚊对溴氰菊酯、氟氯氰菊酯、DDT和马拉硫磷抗药性,为合理使用杀虫剂提供科学依据。方法采用WHO区分剂量标准和方法,观察首只蚊虫击倒时间、击倒率和死亡率,根据校正死亡率判定抗性级别。结果襄州区、广水市、京山县中华按蚊对DDT、溴氰菊酯和氟氯氰菊酯首只蚊虫击倒时间依次为4～42 min、4～20 min和5～18 min,击倒率依次为2.06%～6.60%、4.9%～11.43%和3.23%～23.47%,校正死亡率均小于50%,均为抗性群体;对马拉硫磷首只蚊虫击倒时间10～13 min,击倒率17.76%～35.05%,校正死亡率广水市小于80%为抗性群体、襄州区和京山县在82.6%～88.78%,为初步抗性群体。结论湖北省部分地区中华按蚊对DDT仍具有很高的抗药性,对溴氰菊酯和氟氯氰菊酯也具有很强的抗药性和抗击倒力。应加强监测,现场防制宜适当加大使用剂量,确保防制效果。
Objective To understand the resistance of Anopheles sinensis to deltamethrin, cyfluthrin, DDT and malathion and to provide a scientific basis for the rational use of insecticides. Methods WHO divided dose criteria and methods to observe the first mosquito knockdown time, knockdown rate and mortality, according to the adjusted mortality rate to determine the level of resistance. Results The first mosquitoes of DDT, deltamethrin and cyfluthrin in Xiangzhou District, Guangshui City and Jingshan County were knocked out for 4 ~ 42 min, 4 ~ 20 min and 5 ~ 18 min, respectively. The knockdown rate Followed by 2.06% -6.60%, 4.9% ~ 11.43% and 3.23% ~ 23.47% respectively, and the corrected mortality rates were less than 50%, all of them were resistant groups. The first mosquito killing time of malathion was 10 ~ 13 min, The knockout rates ranged from 17.76% to 35.05%, and the corrected mortality rate was less than 80% in Guangshui City as resistant populations. Xiangzhou District and Jingshan County were between 82.6% and 88.78%, being the initial resistant population. Conclusion Anopheles sinensis is still highly resistant to DDT in some areas of Hubei Province, and resistant to deltamethrin and cyfluthrin. Should be strengthened monitoring, on-site prevention should be appropriate to increase the use of dose to ensure that the control effect.