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在幼穗发育的雌、雄蕊形成期 ,用化学杀雄剂 号 (30 mg/ m2 )处理水稻后 ,研究其幼穗、颖花 ,花药中蛋白质和核酸的代谢特点。在处理后第 5天的幼穗中 ,核酸和蛋白质含量明显低于对照 ,而相应水解酶活性明显高于对照。随幼穗发育 ,这种特点越来越显著 ,至处理后第 15天 ,花药中核酸总量、DNA、RNA含量和蛋白质含量比对照低 4 0 %以上 ,而相应水解酶活性比对照高达 4 6%以上。这些结果显示化学杀雄剂 号处理能使核酸和蛋白质严重匮乏 ,这可能是诱导水稻雄性不育的生化基础。
During the formation of female and stamens, the metabolic characteristics of young panicle, spikelet and anther protein and nucleic acid were studied after the rice was treated with chemical maleicide (30 mg / m2). In young panicle on the fifth day after treatment, the content of nucleic acid and protein was significantly lower than that of the control, while the corresponding hydrolase activity was significantly higher than that of the control. With the development of young panicles, this characteristic became more and more obvious. By the 15th day after treatment, the total amount of nucleic acid, DNA, RNA content and protein content in anther were 40% lower than that in the control, while the corresponding hydrolase activity was up to 4 6% or more. These results show that the chemical treatment of maleicide can make the nucleic acid and protein severely deficient, which may be the biochemical basis of inducing male sterility in rice.