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我们在科研工作中摸索出一种显示尼氏体的简单方法,对采用4％多聚甲醛(用10％甲醛或95％乙醇固定均可)固定的鸡胚背根节细胞悬液的涂片,用苏本精染色,清晰地显示了神经细胞浆内的嗜碱性物质-尼氏体。1 材料和方法 Hamburger 35期来亨鸡胚,摘取腰段背根节,用0.125％胰蛋白酶消化(37℃)30分钟,打散成细胞悬液后涂片,稍干后用4％多聚甲醛固定10分钟,自来水冲洗至无福尔马林气味后,用苏木精染色10分钟,用1％盐酸酒精分色90秒钟,常规脱水、透明、封片后置显微镜下观察。
In our research work, we found out a simple way to display the microstructure of Nissl. Using 4% paraformaldehyde (fixed with 10% formaldehyde or 95% ethanol) , Stained with sulbenin, clearly shows the basophilic substance - Nissl in the neuronal cytoplasm. Materials and methods Hamburger35 to Laiheng chicken embryo, remove the lumbar dorsal root ganglia, with 0.125% trypsin digestion (37 ℃) for 30 minutes, break up into cell suspension after smear, slightly dry with more than 4% POM was fixed for 10 minutes, rinsed with water until no formalin was obtained, stained with hematoxylin for 10 minutes, and separated with 1% hydrochloric acid for 90 seconds. Conventional dehydration and transparency were observed under a microscope.