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克氏铁琼脂(Kliger Iron Agar)培养基在鉴定急性肠道感染病原菌时使用最广,用以测定供检菌对乳精、葡萄糖的发酵及硫化氢产生能力。此培养基对那些H_2S产生能力微弱的肠道菌(如某些沙门氏菌)的检出率很低。为提高对这类细菌检出的阳性率,作者提出将克氏培养基中通常用以产生H_2S的基质硫代硫酸钠(Na_S_2O_3)改为重亚硫酸钠(Na_S_2O_5),将H_2S生成的指示剂硫酸亚铁(FeSO_4)
Kliger Iron Agar medium is the most widely used in the identification of pathogens for acute enteric infections and is used to determine the ability of the test bacteria to ferment lactate and glucose and produce hydrogen sulfide. This medium has a low detectable rate of enterobacteria (eg, some Salmonella) that are weakly capable of H 2 S production. In order to improve the detection rate of these bacteria, the authors proposed to change the sodium thiosulfate (Na_S_2O_3) which is commonly used to produce H_2S in Kirschner series into sodium sulfite (Na_S_2O_5) Iron (FeSO_4)