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用常规平板法分析杂交瘤上清液既浪费时间,又需要大量的材料,而且在使用原始上清液前还需要浓缩.K?ohler(1980年)的夹心法要求待测定的杂交瘤上清液必须浓缩,相关的抗原一定是有效力的,并且能粘着于聚合物的表面,其他方法也很浪费时间.本文所介绍的固相放射免疫试验,在不浓缩、不纯化的杂交瘤上清液中能检测出免疫球蛋白的类和亚类,而且不用抗原.本法是一种免疫夹心法,其要点是:(1)用免疫蛋白类或亚类特异性抗血清IgG包被微量板孔聚合物表面,(2)加不纯化、不浓缩的杂交瘤上清液,(3)再加~(125)Ⅰ标记的兔抗小鼠IgG的抗体,(4)最后计数cpm.
Analysis of the hybridoma supernatant by conventional plate methods is time-consuming and requires a large amount of material and requires concentration prior to use of the original supernatant.K? Ohler (1980) sandwich method requires that the hybridoma supernatant to be assayed Liquid must be concentrated, the relevant antigen must be effective, and can be adhered to the surface of the polymer, other methods are also a waste of time.The solid phase radioimmunoassay described in this article, not concentrated, impure hybridoma supernatant Liquid can detect immunoglobulin class and subclass, and without antigen.This method is an immune sandwich method, the main points are: (1) with immunoproteins or subclasses specific anti-serum IgG coated microplate Pore polymer surface, (2) adding an unpurified, non-concentrated hybridoma supernatant, (3) adding a ~ (125) I labeled rabbit anti-mouse IgG antibody, and (4) finally counting cpm.