【摘 要】
：
针对出口家禽新城疫泄殖腔棉拭样品病毒检测的需要,建立了PCR和ELISA联合检测方法.结果表明:检测时间较常规缩短7～14 d;通过被检样品鸡胚传代,提高了阳性检测率;特异性强,只与
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针对出口家禽新城疫泄殖腔棉拭样品病毒检测的需要,建立了PCR和ELISA联合检测方法.结果表明:检测时间较常规缩短7～14 d;通过被检样品鸡胚传代,提高了阳性检测率;特异性强,只与不同类型新城疫病毒反应,不与其他常见禽类病毒反应;所检的3 216个样本,符合率98.8%,用多重RT-PCR和脑内接种试验两种方法鉴定的114份样品,符合率99.1%,与GenBank中部分新城疫病毒F基因比较,同源性81%～100%.
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