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目的：比较研究卵巢恶性肿瘤ｐ５３基因第５，６，７外显子突变与ｐ５３基因蛋白的过度表达和临床生物学行为的相关性．方法：对５０例卵巢恶性肿瘤及１０例良性肿瘤石蜡切片进行p５３蛋白免疫组化检测，其中２３例卵巢恶性肿瘤的新鲜标本进行了ｐ５３基因第５，６，７外显子ＰＣＲ－ＳＳＣＰ检测．结果：５０例卵巢恶性肿瘤中１２例Ｐ５３蛋白阳性表达２４％（１２／５０）．上皮癌２９％（９／２８）生殖细胞肿瘤１０％（１／１０）间质细胞瘤２５％（２／８），转移瘤２５％（１／４）在２３例标本ＰＣＲ－ＳＳＣＰ检测中突变率为３５％（８／２３）第５，６．７外显子突变率分别为９％（２／２３），９％（２／２３）．１７％（４／２３）．两者均阳性者为２例．即ＰＣＲ－ＳＳＣＰ检测和蛋白表达均阳性第６．７外显子各１例．结论：①卵巢恶性肿瘤存在ｐ５３基因突变蛋白的过度表达．但突变与蛋白阳性表达并非完全一致；②ｐ５３基因突变及其蛋白过度表达与卵巢恶性肿瘤组织类型和临床分期无明显相关性
OBJECTIVE: To compare the correlation between mutations in exons 5, 6 and 7 of p53 gene and p53 gene protein overexpression and clinical biological behavior in ovarian cancer. Methods: Fifty cases of ovarian malignant tumors and 10 cases of benign tumor paraffin sections were examined by immunohistochemistry. P53 was detected by PCR-SSCP in exon 5, 6 and 7 of 23 fresh ovarian cancer samples. Results: The positive expression of P53 protein in 12 cases of ovarian malignant tumors was 24% (12/50). Epithelial neoplasia 29% (9/28) Germ cell tumors 10% (1/10) Smell tumor 25% (2/8), metastases 25% (1/4) Mutation in PCR-SSCP detection of 23 specimens The rate of exon 5 and 6 in exon 35% (8/23) was 9% (2/23) and 9% (2/23), respectively. 17% (4/23). Both were positive in 2 cases. That PCR-SSCP detection and protein expression were positive in 6.7 exons in 1 case. Conclusions: ① Overexpression of p53 gene mutation exists in ovarian cancer. However, the mutation was not completely consistent with the protein positive expression. (2) There was no significant correlation between the mutation of p53 gene and its overexpression and the tissue type and clinical stage of ovarian malignant tumor