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对无环鸟苷和鸟嘌呤的高效毛细管电泳（HPCE）分离测定条件进行了详细研究，建立了以2－氨基－5－巯基－3，4－噻二唑为内标的快速、灵敏的HPCE定量分析方法。当无环鸟苷和鸟嘌呤的浓度分别在10～300mg／L和5～300mg／L范围时，可进行定量分析，最低检出限分别为8．52mg／L和2．80mg／L。该方法用于无环鸟苷产品的质量检测，结果准确。用于尿液中的无环鸟苷和鸟嘌呤的快速测定，相对标准偏差小于4．5％。无环鸟苷和鸟嘌呤的回收率分别优于81％和86％。
The conditions for the high performance capillary electrophoresis (HPCE) separation of acyclovir and guanine were studied in detail. A fast and sensitive quantification of HPCE with 2-amino-5-mercapto-3,4-thiadiazole as internal standard was established Analytical method. When the concentration of acyclovir and guanine were in the range of 10 ~ 300mg / L and 5 ~ 300mg / L, the quantitative analysis was carried out with the minimum detection limits of 8.52mg / L and 2.80mg / L, respectively. The method for the acyclovir product quality testing, the results are accurate. For the rapid determination of acyclovir and guanine in urine, the relative standard deviation was less than 4.5%. The recoveries of acyclovir and guanine were better than 81% and 86%, respectively.