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利用SRAP分子标记构建了14份不同地理来源、表型具有差异的油莎豆品系的分子指纹图谱并进行遗传多样性分析。结果表明100对引物中共有多态性引物42对,扩增出多态性带328条,平均每对引物7.8条。28对引物在12个品系上具有特征谱带,除品系4和14外,均可用1对引物进行鉴定;采用引物组合法仅用Me2/Em6和Me8/Em11这2对引物就可将14份材料区分开,并利用这2对引物构建了上述品系的数字指纹图谱。UPGMA聚类分析表明,所有参试材料间的遗传距离在0.12～0.75之间,平均为0.42,表明我国不同地理来源的油莎豆品系遗传差异较大,具有较为丰富的遗传多样性。
Using SRAP molecular markers, 14 fingerprints of oilseed rape cultivars with different geographical origins and different phenotypes were constructed and their genetic diversity was analyzed. The results showed that there were 42 pairs of polymorphic primers among 100 pairs of primers, and 328 polymorphic bands were amplified, with an average of 7.8 pairs per primer. 28 pairs of primers had characteristic bands in 12 lines, except for lines 4 and 14, one pair of primers could be used for identification. Only two pairs of primers, Me2 / Em6 and Me8 / Em11, The materials were distinguished and the digital fingerprints of the above lines were constructed using these two pairs of primers. UPGMA cluster analysis showed that the genetic distance between all the tested materials ranged from 0.12 to 0.75, with an average of 0.42, indicating that the genetic diversity of the oil sambucus lines of different geographical origins in China is quite large and has a rich genetic diversity.