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目的:通过动物实验,从肿瘤坏死因子α水平变化角度探讨活血药对正常妊娠结局的影响及机理。方法:将日龄为42～56天SPF级Wistar大鼠雌雄配对后筛选40只孕鼠,随机分4小组:对照组及低、中、高剂量组,每组10只,统一给药灌胃,每天1次,持续7天,观察妊娠情况,于妊娠第18～19日开胸一次处死大鼠,采血采用放免法测定血清TNF-α浓度,解剖后取出双侧子宫置于干净弯盘内,立即数双侧子宫着床点数以及活胎数。将子宫、胎盘用生理盐水冲冼干净,匀浆、过滤后取液体用放免法测定TNF-α浓度。结论:低剂量中药佛手散对正常妊娠大鼠的妊娠结局亦无不良影响,但当剂量达到一定程度时,则会升高血清及局部组织的引起肿瘤坏死因子α,使正常妊娠大鼠自然流产。
OBJECTIVE: To investigate the effect and mechanism of Huoxue Pills on normal pregnancy outcomes from the perspective of changes in tumor necrosis factor alpha levels in animal experiments. METHODS: Forty-five pregnant rats of SPF grade Wistar rats aged 42-56 days were selected and randomly assigned to 4 groups: control group and low, middle, and high dose groups, 10 in each group. , Once a day, for 7 days, observe the pregnancy, start the rats on the 18th to the 19th day of gestation, and then sacrifice the rats. Blood samples were taken to measure the concentration of serum TNF-α. After dissection, both sides of the uterus were removed and placed in a clean curved dish. , immediately count the number of bilateral uterine implantation points and the number of live births. The uterus and placenta were flushed with physiological saline, homogenized, filtered, and the liquid was taken for determination of TNF-α concentration by radioimmunoassay. Conclusion: Low-dose traditional Chinese medicine Foshou Powder has no adverse effect on the pregnancy outcome in normal pregnant rats. However, when the dose reaches a certain level, serum and local tissue causes tumor necrosis factor alpha to increase, leading to spontaneous abortion in normal pregnant rats. .