论文部分内容阅读
1 材料和方法 Wistar大鼠28只,随机分成两组,正常对照组、糖尿病组各14只,糖尿病组动物在实验开始时从阴茎背静脉注尿菌链霉素,以破坏胰岛B细胞,制成糖尿病模型。于实验第12周末处死,取胰腺等等织,用Bouin固定液固定,石蜡切片(2至3μm厚),作下述染色,进行组织学观察。 2 染色方法 ①切片脱蜡水洗,蒸馏水洗。②酸性高锰酸钾氧化3min。③流水冲洗约1min。④2％草酸漂白,而后流水冲洗5min,蒸馏水洗1次。⑤60％洒精过1次。③染醛复红液浸泡9分钟左右。其配方
1Materials and Methods 28 Wistar rats were randomly divided into two groups, normal control group and diabetes group. Fourteen rats in the diabetic group were injected with streptozotocin from the back of penis at the beginning of the experiment to destroy islet B cells. Become a diabetic model. At the end of the experiment, the rats were sacrificed at the end of the 12th week and the pancreas and the like were excised, fixed with Bouin’s fixative solution, paraffin sections (2 to 3 μm thick), and stained as described below for histological observation. 2 staining method 1 slice dewaxed water washing, distilled water washing. 2 acidic potassium permanganate oxidation 3min. 3 water rinse about 1min. 42% oxalic acid was bleached, washed with water for 5 minutes, and washed with distilled water once. 560% Sprinkle fine one time. 3 dyed aldehyde rehydration solution soaked for about 9 minutes. Its formula