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目的:建立分光光度法测定乳与乳制品中硫氰酸盐含量的方法。方法:样品经处理后,在中性介质中,于50℃条件下,样品中硫氰酸根与氯胺T反应生成氯化氰,再与异烟酸作用,经水解后生成戊烯二醛,最后与吡唑啉酮缩合生成蓝色染料,该蓝色染料的吸光度与硫氰酸根的浓度在一定范围内呈线性关系,以此测定样品中硫氰酸根的浓度。结果:该方法硫氰酸盐浓度在0.0 mg/L～1.0 mg/L呈线性关系,相关系数为0.9997,检出限为0.02 mg/kg,相对标准偏差在3.48%,样品加标回收率为90.8%。结论:该法操作简单,灵敏度高,准确可靠,适用于乳制品的质量监测。
Objective: To establish a spectrophotometric method for the determination of thiocyanate content in milk and dairy products. Methods: The sample was treated in a neutral medium at 50 ℃, the sample thiocyanate and chloramine T reaction of cyanogen chloride, and then with isonicotinic acid role, after hydrolysis generated glutaraldehyde, Finally, pyrazolone condensation generated blue dye, the blue dye absorbance and concentration of thiocyanate in a certain range of linear relationship, in order to determine the concentration of thiocyanate in the sample. Results: The concentration of thiocyanate in the range of 0.0 mg / L to 1.0 mg / L showed a linear relationship with a correlation coefficient of 0.9997, a detection limit of 0.02 mg / kg and a relative standard deviation of 3.48%. The spiked recoveries 90.8%. Conclusion: The method is simple, sensitive, accurate and reliable, suitable for the quality monitoring of dairy products.