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目的研究补肾壮骨颗粒对维甲酸致骨质疏松大鼠骨组织计量学的影响,探讨补肾壮骨颗粒防治骨质疏松症的机制。方法实验选用3月龄SD大鼠,按体重随机分为5组:正常对照组、骨质疏松模型组、补肾壮骨颗粒高低剂量组、仙灵骨葆阳性对照组。各组大鼠上午均灌胃给予维甲酸2周,造模同时下午按上述剂量灌胃给予受试药3周,末次给药后晚上禁食,次日以10%水合氯醛腹腔注射麻醉动物,腹主动脉放血处死动物后取左右胫骨,并去掉附着的软组织,放入10%福尔马林中固定,移入10%EDTA溶液中脱钙,再转入酒精内脱水,石蜡包埋,切片并行H-E染色。在光镜10×10倍视野下应用高清晰度彩色病理图文分析系统对胫骨上段松质骨的骨组织作静态测量并计算骨小梁面积百分数、骨小梁厚度、骨小梁数量和骨小梁分离度(取切片中心部的五个视野测量并取其平均数),观察胫骨上段病理形态学变化。结果与正常对照组相比,模型对照组大鼠的胫骨近端骨松质骨小梁面积百分数及厚度减少,骨小梁数量及分离度增加,有统计学意义(P<0.01),说明造模成功;与模型对照组相比,补肾壮骨颗粒组胫骨近端骨松质的骨小梁面积百分数及厚度增加,骨小梁数量及分离度减少,有统计学意义(P<0.01)。结论补肾壮骨颗粒能减少维甲酸通过激活破骨细胞所引起的骨丢失,改善骨小梁的形态,起到防治骨质疏松症的作用。
Objective To study the effect of Bushenzhuanggu Granules on bone histomorphometry in rats with osteoporosis induced by retinoic acid and to explore the mechanism of Bushenzhuanggu Granules in preventing and treating osteoporosis. Methods Three-month-old Sprague-Dawley rats were randomly divided into 5 groups: normal control group, osteoporosis model group, high dose group of Bushenzhuanggu Granules, and positive control group of Xianlingguchi. Rats in each group were intragastrically administered with retinoic acid for 2 weeks in the morning. At the same time, the mice were given intragastric administration of the test drug at the same dose in the afternoon for 3 weeks. Fasting was performed at night after the last administration. The animals were anesthetized with 10% chloral hydrate intraperitoneally the next day. After the animal was sacrificed by abdominal aorta bleeding, the left and right iliac bones were removed, and the attached soft tissue was removed and fixed in 10% formalin, transferred to 10% EDTA solution for decalcification, and then transferred to alcohol for dehydration, paraffin embedding, and slice parallel HE. dyeing. A high-resolution color pathology analysis system was used to statically measure the bone tissue of the upper tibial cancellous bone under a 10×10 field view of the light microscope and the trabecular area percentage, trabecular thickness, trabecular bone number, and bone were calculated. The degree of trabecular separation (taken from the five fields measured at the center of the slice and taken as the average) was used to observe the pathological changes of the upper segment of the humerus. Results Compared with the normal control group, the percentage of the trabecular bone area and the thickness of the proximal humerus in the model control group decreased, and the number and the degree of the trabecular bone increased. There was a statistically significant difference (P<0.01). The model was successful; compared with the model control group, the percentage and thickness of trabecular bone area in the proximal humerus of the Bushenzhuanggu granule group increased, and the number and degree of trabecular bone trabecula decreased, which was statistically significant (P<0.01). Conclusion Bushen Zhuanggu granule can reduce the bone loss caused by activation of osteoclasts by retinoic acid, improve the morphology of trabecular bone, and play a role in the prevention and treatment of osteoporosis.