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在神经系统的生长发育过程中 ,星形胶质细胞对神经元生长发育的作用是一项重要的研究课题。本文以体外培养的 SD大鼠大脑皮质星形胶质细胞与 PC12神经元按不同细胞数目比例 ( 5 0∶ 1～ 1∶ 1)共同培养 ,并用其制备的条件培养液培养 PC12细胞 ,用快速灵敏的 MTT比色法测定 PC12神经元的细胞活力 ,用光学相差显微镜观察 PC12细胞形态学变化。结果显示 ,星形胶质细胞条件培养液可增强 PC12细胞活力 ( MTT测定的 OD值由 0 .2 5 5± 0 .0 12提高到 0 .5 10± 0 .0 3 6,P<0 .0 0 1,且细胞折光性较对照组强 ) ,却不能促使 PC12神经元突起的生出。将星形胶质细胞与 PC12细胞按 3 0∶ 1～ 1∶ 1的比例共同培养时 ,既可提高 PC12细胞折光性和光晕又可促使其突起的生长 ;如按 5 0∶ 1～ 40∶ 1的比例共同培养时 ,只观察到提高 PC12细胞折光性和光晕 ,而无促使其突起生长发育的作用。本文结果提示 ,PC12神经元细胞活力的提高与星形胶质细胞分泌到条件培养液中的可溶性因子有关 ,而 PC12神经元突起生长发育可能是和与星形胶质细胞的直接接触以及二者的细胞数目比有关。
The role of astrocytes in neuronal growth and development during the development of the nervous system is an important research topic. In this paper, cultured in vitro SD rat cortical astrocytes and PC12 neurons in different cell number ratio (50: 1 ~ 1: 1) co-culture, and conditioned medium with its preparation of PC12 cells cultured with fast The cell viability of PC12 neurons was detected by sensitive MTT colorimetric method. Morphological changes of PC12 cells were observed by optical phase contrast microscope. The results showed that astrocyte conditioned medium could enhance the viability of PC12 cells. The OD value of MTT assay increased from 0.525 ± 0.012 to 0.510 ± 0.306, P <0. 0 0 1, and the cell refraction than the control group), but can not promote the process of PC12 neuron protrusion. When astrocytes and PC12 cells are co-cultured in the ratio of 30: 1 to 1: 1, the PC12 cells can enhance the refraction and the halo and promote the growth of the cells. For example, 1 ratio co-cultured, only to improve the PC12 cell refraction and halo, without promoting its role in the process of protruding growth and development. Our results suggest that increased viability of PC12 neurons is associated with soluble factors that are secreted by astrocytes into conditioned media and that PC12 neuronal growth may be related to direct contact with astrocytes and both The number of cells than the relevant.