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目的观察失血条件下氰类毒剂中毒对大鼠肺换气功能及磷脂含量的影响。方法将健康成年SD大鼠随机分为假手术组、失血性休克组、氰化钾中毒组和失血性休克复合氰化钾中毒组,每组6只动物。各实验组设15、30、60和120min 4个时相点,检测大鼠肺换气功能的变化,采用伊文思蓝(Evan’s blue,EB)染色观察肺血管通透性的改变以及定磷法测定肺组织中磷脂总量(total phospholipids,TPL)。结果与氰化钾中毒组比较,失血条件下氰化钾中毒大鼠肺泡动脉血氧分压差(PAaDO2)和呼吸指数(RI)不同程度升高,大鼠肺部血管通透性增强以及肺磷脂含量明显降低,差异有统计学意义(P<0.05)。结论战(创)伤失血明显加重氰类毒剂对肺功能的损伤效应。
Objective To observe the effects of cyanogen poisoning on pulmonary ventilation and phospholipid content in rats under blood loss. Methods Healthy adult SD rats were randomly divided into sham operation group, hemorrhagic shock group, potassium cyanide poisoning group and hemorrhagic shock combined with potassium cyanide poisoning group, 6 animals in each group. Each experimental group was set at 4, 15, 30, 60 and 120 min intervals to detect the change of lung ventilation function in rats. Evan’s blue staining was used to observe the change of pulmonary vascular permeability and the determination of phosphorus Total phospholipids (TPL) in lung tissue were measured. Results Compared with potassium cyanide poisoning group, alveolar arterial partial pressure of oxygen (PAaDO2) and respiratory index (RI) of potassium cyanide poisoning rats were increased to different extents, pulmonary vascular permeability increased and lung Phospholipid content was significantly lower, the difference was statistically significant (P <0.05). Conclusions Combat (wound) blood loss significantly aggravates the damaging effects of cyanotoxic agents on lung function.