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详细研究了亚氨基二乙酸（ＩＤＡ）在硅胶基质上键合的适宜条件，利用国产小颗粒大孔径硅胶（７μｍ，３０ｎｍ）制备了固定有Ｃｕ２＋，Ｎｉ２＋，Ｃｏ２＋，Ｚｎ２＋的金属螯合吸附剂，比较了蛋白质在金属螯合柱和裸柱上的保留特性，利用合成的Ｃｕ－ＩＤＡ－硅胶柱分离了来自牛血红细胞的超氧化物歧化酶（ＳＯＤ）。
The suitable conditions for the bonding of IDA on silica gel substrate were studied in detail. Metal chelate adsorbents with Cu2 +, Ni2 +, Co2 + and Zn2 + were immobilized on small-size large-pore silica gel (7μm, 30nm) The retention properties of proteins on metal chelates and bare columns were compared. Superoxide dismutase (SOD) from bovine red blood cells was isolated using a synthetic Cu-IDA-silica gel column.