论文部分内容阅读
目的分析214例无精子及少精子症患者的染色体核型和生殖激素水平,探讨男性不育患者的遗传学因素。方法采用外周血培养及G带染色分析214例无精子及少精子症患者的染色体核型,电化学发光法测定其血清生殖激素水平。结果染色体分析显示,染色体正常核型150例,占样本总量的70.09%;性染色体异核常型39例,占18.22%;染色体多态性变异21例,占9.81%;染色体平衡易位携带者4例,占1.87%。与染色体正常组相比,染色体异常组的卵泡刺激素(FSH)、黄体生成素(LH)和血清泌乳素(PRL)水平显著升高(P<0.05),T值呈降低趋势(P<0.05),E_2无显著性差异(P>0.05)。结论少精子及无精子症与染色体畸变及血清生殖激素异常密切相关,染色体核型及生殖激素的分析和检测,对临床不育症的诊治有指导意义。
Objective To analyze the karyotype and reproductive hormone levels in 214 patients with azoospermia and oligospermia, and to explore the genetic factors of male infertility. Methods The karyotypes of 214 azoospermia and oligospermia patients were analyzed by peripheral blood culture and G-band staining. The levels of serum reproductive hormones were determined by electrochemiluminescence. Results Chromosome analysis showed that there were 150 cases of normal karyotype in chromosomes, accounting for 70.09% of the total sample size, 39 cases of sex chromosome abnormalities in common type (18.22%), 21 cases of chromosome polymorphism variation (9.81%), chromosome balance translocation 4 cases, accounting for 1.87%. The levels of follicle stimulating hormone (FSH), luteinizing hormone (LH) and serum prolactin (PRL) in patients with chromosomal abnormalities were significantly higher than those in normal controls (P <0.05) ), E_2 no significant difference (P> 0.05). Conclusion Less sperm and azoospermia are closely related to chromosomal aberrations and serum reproductive hormones abnormalities. Analysis and detection of chromosomal karyotypes and reproductive hormones are instructive in the diagnosis and treatment of infertility.