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目的研究survivin各转录变异体在胃癌组织中的表达情况及与其预后的关系。方法采用实时荧光定量RT-PCR技术,对77例胃恶性肿瘤患者的肿瘤组织、正常胃黏膜的成对标本进行mRNA定量检测。结果在检测患者肿瘤组织中野生型survivin、survivin-2B与survivin-△Ex3的表达量均显著高于其对应正常胃黏膜组织中的表达量(P<0.01)。在胃癌组织中,survivin的表达率为100%(77/77),survivin-2B的表达率为79.8%(61/77),survivin-△Ex3的表达率为64.9%(50/77)。survivin高表达组患者的生存率明显低于低表达组(P<0.01)。结论Survivin的3个转录变异体中,野生型survivin对胃癌患者预后具有重要的评估价值。
Objective To investigate the expression of survivin transcripts in gastric carcinoma and its relationship with prognosis. Methods The real-time fluorescence quantitative RT-PCR technique was used to detect the mRNA of 77 gastric cancer specimens from normal gastric mucosa. Results The expression levels of survivin-survivin-2B and survivin-△ Ex3 in tumor tissue were significantly higher than those in normal gastric mucosa (P <0.01). The expression of survivin in gastric cancer was 100% (77/77), the expression of survivin-2B was 79.8% (61/77), and the expression of survivin- △ Ex3 was 64.9% (50/77). Survivin high expression group patients survival rate was significantly lower than the low expression group (P <0.01). Conclusion Among the three transcriptional variants of Survivin, wild-type survivin has an important value in the prognosis of patients with gastric cancer.