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目的观察弓形虫可溶性速殖子抗原(STAg)联合IFNγ滴鼻免疫BALB/c小鼠诱导的免疫应答,为研制弓形虫黏膜疫苗提供实验依据。方法将BALB/c小鼠随机分为实验组和对照组,实验组以STAg(20μg/只)+IFNγ(1 000 U/只)滴鼻,对照组以PBS滴鼻。免疫2次,间隔2周。分别于初次免疫后0、2、4、6、8、10、12周处死小鼠,分离脾淋巴细胞、肠上皮内淋巴细胞(IELs)并计数;分离血清,用ELISA法测定IgA和IgG含量。结果免疫后实验组小鼠IELs和脾淋巴细胞均有增生,IELs和脾淋巴细胞数量均于初次免疫后4周达峰值,IEL数量4、6周显著高于对照组;脾淋巴细胞数量2、4周显著高于对照组。实验组小鼠血清IgG和IgA水平均于初次免疫后4周达峰值,IgG水平2、4周显著高于对照组,至12周时仍高于对照组;IgA水平4、6周显著高于对照组。结论STAg联合IFNγ滴鼻免疫BALB/c小鼠,可有效诱导黏膜及系统免疫应答,且可持续较长时间。
Objective To observe the immune response induced by Tachyzoite soluble tachyzoite antigen (STAg) combined with IFNγ intranasal immunization of BALB / c mice and provide experimental basis for the development of Toxoplasma gondii mucosal vaccine. Methods BALB / c mice were randomly divided into experimental group and control group. The experimental group was intranasally administrated with STAg (20μg / mouse) + IFNγ (1 000 U / mouse), while the control group was dripped with PBS. Immunization 2 times, an interval of 2 weeks. Mice were sacrificed at 0, 2, 4, 6, 8, 10 and 12 weeks after primary immunization respectively. Splenic lymphocytes and intestinal intraepithelial lymphocytes (IELs) were isolated and counted. Serum levels of IgA and IgG were measured by ELISA . Results After immunization, the number of IELs and splenic lymphocytes in experimental mice were all increased. The number of IELs and splenic lymphocytes reached the peak at 4 weeks after primary immunization. The numbers of IEL at 4 and 6 weeks were significantly higher than those in control group. The number of spleen lymphocytes, 4 weeks was significantly higher than the control group. The levels of serum IgG and IgA in the experimental group reached their peak at 4 weeks after the first immunization, and the levels of IgG at 2 and 4 weeks were significantly higher than those in the control group, and still higher than those in the control group at 12 weeks; IgA levels were significantly higher at 4 and 6 weeks Control group. Conclusion STAg combined with IFNγ intranasal immunization of BALB / c mice can effectively induce mucosal and systemic immune response, and can last a long time.