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目的探讨白三烯通路相关基因多态性与缺血性脑卒中易感性的关系。方法收集2009年1月至2011年1月在南京医科大学第一附属医院住院的缺血性脑卒中患者690例,同时选取同期入院非脑卒中人群690例为对照组,采用PCR-RFLP方法对所有研究对象进行ALOX5 rs2029253 A/G、LTA4H rs6538697 T/C和LTC4S rs730012A/C位点基因型检测,采用Logistic回归分析各位点多态性与缺血性脑卒中发病风险的独立相关性。结果rs730012 C等位基因携带者缺血性脑卒中易感性增高1.37倍(OR=1.37,95%CI 1.08～1.73,P=0.009)。rs2029253 GG基因型降低缺血性脑卒中发病风险(OR=0.72,95%CI 0.55～0.93,P=0.013),rs6538697 CC基因型增加缺血性脑卒中发病风险(OR=1.77,95%CI 1.09～2.89,P=0.022),在调整混杂因素后rs730012多态性与缺血性脑卒中易感性不相关(P>0.05)。结论白三烯通路相关基因多态性可影响缺血性脑卒中的发病风险,具有独立遗传效应。
Objective To investigate the relationship between leukotriene pathway related gene polymorphisms and the susceptibility to ischemic stroke. Methods Six hundred and ninety-nine patients with ischemic stroke admitted to the First Affiliated Hospital of Nanjing Medical University from January 2009 to January 2011 were enrolled. Sixty-nine non-stroke hospitalized patients in the same period were selected as the control group. PCR-RFLP All subjects were genotyped for ALOX5 rs2029253 A / G, LTA4H rs6538697 T / C and LTC4S rs730012A / C loci. Logistic regression was used to analyze the independent association between polymorphisms and ischemic stroke risk. Results The susceptibility to rs730012 C allele was 1.37 times higher in those with ischemic stroke (OR = 1.37, 95% CI 1.08-1.73, P = 0.009). The rs2029253 GG genotype reduced the risk of ischemic stroke (OR = 0.72, 95% CI 0.55-0.93, P = 0.013), and genotype CC of rs6538697 increased the risk of ischemic stroke (OR = 1.77, 95% CI 1.09 ~ 2.89, P = 0.022). There was no correlation between rs730012 polymorphism and susceptibility to ischemic stroke after adjusting confounding factors (P> 0.05). Conclusion Leukotriene pathway-related gene polymorphisms can affect the risk of ischemic stroke and have an independent genetic effect.