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体外培养3代融合的牛视网膜血管周细胞,分别在对照组(5.5mmol/L)与高糖各组(15,25,35mmol/L)中孵育6d,透射电镜观察周细胞超微结构改变,硫代巴比妥酸法检测培养液丙二醛(MDA)水平;黄嘌呤氧化酶反应系统检测培养液超氧化物歧化酶(SOD)水平;单细胞凝胶电泳检测DNA损伤情况。结果与对照组相比,高糖各组MDA含量/SOD活力比值增加(P<0.01)。高糖各组周细胞彗尾长度及拖尾率均增高,差异有统计学意义(P<0.01),相同剂量组周细胞彗尾长度与MDA/SOD比值呈正相关(r=0.729,P<0.01)。结论:高糖可引起牛视网膜毛细血管周细胞氧化应激和DNA损伤,氧化应激可能在高糖诱导的牛视网膜毛细血管周细胞DNA损伤中起重要作用。
The cultured bovine retinal pericytes were cultured in vitro for 6 days in the control group (5.5mmol / L) and high glucose (15,25,35mmol / L) for 6 days respectively. The ultrastructural changes of the pericytes were observed by transmission electron microscope. Thiobarbituric acid method was used to detect the level of malondialdehyde (MDA) in the culture medium; the level of superoxide dismutase (SOD) was detected by xanthine oxidase reaction system; DNA damage was detected by single cell gel electrophoresis. Results Compared with the control group, the MDA content / SOD activity increased in each group (P <0.01). The tail length and tailing rate of pericytes in high glucose group were significantly increased (P <0.01), and there was a positive correlation between the tail length and the ratio of MDA / SOD in the same dose group (r = 0.729, P <0.01) ). CONCLUSION: High glucose can cause oxidative stress and DNA damage of bovine retinal capillary pericytes. Oxidative stress may play an important role in high glucose-induced DNA damage of bovine retinal capillary pericytes.