论文部分内容阅读
目的 :探讨日本血吸虫可溶性虫卵抗原(soluble egg antigen,SEA)致敏小鼠骨髓源性树突状细胞(dendritic cells,DCs)后对其细胞表型及Th17细胞分化的影响。方法 :收集BALB/c小鼠骨髓细胞,应用粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)、白介素(interleukin,IL)-4定向诱导小鼠骨髓细胞向DCs分化;流式细胞仪检测SEA刺激培养后DCs表面分子的表达情况;流式细胞术检测DCs分泌IL-6、IL-23的水平,ELISA法检测DCs分泌转化生长因子-β(transforming growth factor-β,TGF-β)的水平;SEA刺激DCs与CD4+T细胞共培养后检测培养上清中IL-6、TGF-β、IL-23和IL-17细胞因子水平。结果:诱导培养出可供实验用的高纯度DCs;SEA刺激DCs能表达较高水平的CD80、CD86、CD40、组织相容性复合体(major histocompatibility complex,MHC)-Ⅱ类分子;SEA刺激DCs产生IL-6和TGF-β明显增多;SEA刺激的共培养细胞上清中IL-17水平增高。结论:SEA能够促进DCs成熟,并诱导CD4+T细胞向Th17细胞分化。
Objective: To investigate the effect of soluble egg antigen (SEA) on the phenotype and the differentiation of Th17 cells induced by bone marrow-derived dendritic cells (DCs) in mice. Methods: Bone marrow cells from BALB / c mice were collected and the bone marrow cells were induced to express DCs by granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin (IL) -4 The expression of IL-6 and IL-23 secreted by DCs was detected by flow cytometry and the expression of transforming growth factor-β secreted by DCs was detected by ELISA. TGF-β, TGF-β). The levels of IL-6, TGF-β, IL-23 and IL-17 in the culture supernatants were detected by co-culture of DCs and CD4 + T cells stimulated by SEA. Results: High-purity DCs could be induced and cultured. SEA-stimulated DCs could express higher levels of CD80, CD86, CD40 and MHC class II molecules. SEA-stimulated DCs Producing IL-6 and TGF-β significantly increased; SEA-stimulated IL-17 levels in the supernatant of co-cultured cells increased. Conclusion: SEA can promote the maturation of DCs and induce the differentiation of CD4 + T cells into Th17 cells.