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从菜园土盆栽的黄瓜健壮幼苗根围分离出196 株细菌,用于黄瓜种子的细菌化处理,在光照培养箱中模拟自然状况,首先在平皿中培养初步筛选出30 株促进种子萌发、促进根和下胚轴伸长的菌株;之后用于无菌土盆栽测定,筛选出了能明显提高出苗率,提前出苗时间,增加株高、鲜重等具促生效果的5 株细菌:CN11,CN31,CN45,CN116 和CN129,即为根围促生菌(简称PGPR)。并将5 株PGPR 分别用于接种了引起黄瓜苗期立枯病和猝倒病的三种主要病原菌——立枯丝核菌AG4 (Rhizoctonia solaniAG4)、瓜果腐霉(Pythium aphaniderm atum )、终极腐霉(P. ultim um )后的盆栽测定,发现在促进黄瓜幼苗生长的同时也降低了发病率,与对照相比均达显著水平(P= 0.05),实验结果证明PGPR 促生作用与防病作用具有一致性。
A total of 196 strains of bacteria were isolated from the roots of cucumber seedlings in cucumber garden. The bacteria were used for the bacterial treatment of cucumber seedlings. The natural conditions were simulated in a light incubator. 30 strains were initially screened in a petri dish to promote seed germination and root promotion And hypocotyl stretching strains. After that, it was used in the determination of sterile soil pots and screened out five strains of bacteria which could significantly improve the emergence rate, the emergence time, the plant height, the fresh weight and other growth promoting effects: CN11, CN31 , CN45, CN116 and CN129, that is the root circumference of Probiotics (PGPR). Five PGPRs were used to inoculate Rhizoctonia solani AG4 (Rhizoctonia solani AG4), Pythium aphanidermatum (Pythium aphanidermatum), and the three ultimate pathogenic bacteria The results of pot experiment after P. ultim um showed that the growth of cucumber seedlings was reduced while the growth rate was significantly lower than that of the control (P = 0.05). The experimental results showed that PGPR The role of disease is consistent.