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提出了一种基于SiO2纳米颗粒免疫凝集反应的压电传感器对霍乱毒素的直接检测,研究发现待测物霍乱毒素会同时被晶振表面固定的霍乱毒素抗体和检测体系中SiO2标记的霍乱毒素抗体识别,引起检测体系中的SiO2在传感界面上特异性凝集,从而引起晶振表面的巨大质量变化和检测介质密度和粘度变化。实验结果表明,该方法修饰的探针能够有效地对霍乱毒素进行检测。
A direct detection of cholera toxin based on self-agglutination reaction of SiO 2 nanoparticles was proposed. It was found that the cholera toxin under test could be recognized by the cholera toxin antibody immobilized on the crystal surface and the cholera toxin-labeled antibody in the detection system , Causing the SiO2 in the detection system to agglutinate specifically at the sensing interface, causing a huge mass change on the surface of the crystal and detecting the change of the density and the viscosity of the medium. The experimental results show that the probe modified by this method can effectively detect cholera toxin.