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Aim:To assess the inhibitory effect of agmatine on tumor growth in vivo andtumor cell proliferation in vitro.Methods:The transplanted animal model,[~3H]thymidine incorporation assay,3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazo-lium assay,and lactate dehydrogenase(LDH)release assay were performed.Results:Agmatine,at doses of 5-40 mg/kg,suppressed the S_(180)sarcoma tumorgrowth dose-dependently in mice in vivo and the highest inhibitory ratio reached31.3% in Kunming mice and 50.0% in Balb/c mice,respectively.Similar resultswere obtained in the transplanted B_(16)melanoma tumor model.Agmatine(1-1000 μmol/L)was able to attenuate the proliferation of cultured MCF-7 humanbreast cancer cells in vitro in a concentration-dependent manner and the highestinhibitory ratio reached 50.3% in the[~3H]thymidine incorporation assay.Additionally,in the LDH release assay,spermine(20μmol/L)and spermidine(20μmol/L)increased the LDH release significantly,but agmatine(1-1000μmol/L)did not,indicating that the inhibitory effect of agmatine on the proliferation ofMCF was not related to cellular toxicity.In the[~3H]thymidine incorporation assay,putrescine(12.5-100.0μmol/L)could reverse the inhibitory effect of agmatine onthe proliferation of MCF concentration-dependently,suggesting that the inhibi-tory effect of agmatine on the proliferation of MCF might be associated with adecreased level of the intracellular polyamines pool.Conclusion:Agmatine hadsignificant inhibitory effect on transplanted tumor growth in vivo and prolifera-tion of tumor cells in vitro,and the mechanism might be a result of inducingdecrease of intracellular polyamine contents.
Aim: To assess the inhibitory effect of agmatine on tumor growth in vivo and tumor cell proliferation in vitro. Methods: The transplanted animal model, [~ 3H] thymidine incorporation assay, 3- [4,5-dimethythiazol-2-yl] , 5-diphenyltetrazo-lium assay, and lactate dehydrogenase (LDH) release assay were performed. Results: Agmatine, at doses of 5-40 mg / kg, suppressed the S_ (180) sarcoma tumorgrowth dose-dependently in mice in vivo and the highest inhibitory ratio reached 31.3% in Kunming mice and 50.0% in Balb / c mice, respectively.Similar resultswere obtained in the transplanted B_ (16) melanoma tumor model.Agmatine (1-1000 μmol / L) was able to attenuate the proliferation of cultured MCF-7 human breast cancer cells in vitro in a concentration-dependent manner and the highest inhibition ratio reached 50.3% in the [~ 3H] thymidine incorporation assay. Additionally, in the LDH release assay, spermine (20 μmol / L) and spermidine 20 μmol / L) increased the LDH release significantly, but agmatine (1-1000 μmol / L) did not, indicating th at inhibitory effect of agmatine on the proliferation of MCF was not related to cellular toxicity. the [~ 3H] thymidine incorporation assay, putrescine (12.5-100.0 μmol / L) could reverse the inhibitory effect of agmatine on the proliferation of MCF concentration- dependently , suggesting that the inhibi-tory effect of agmatine on the proliferation of MCF might be associated with adecreased level of the intracellular polyamines pool. Confluence: Agmatine hadsignificant inhibitory effect on transplanted tumor growth in vivo and prolifera-tion of tumor cells in vitro, and the mechanism might be a result of inducingcrease of intracellular polyamine contents.