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采用双池培养系统研究颗粒细胞（Ｇ－Ｃ）与内泡膜细胞（Ｔ－Ｃ）在甾体激素生成中的旁分泌调节作用。实验选用小母猪的卵巢组织，分离出Ｇ－Ｃ和Ｔ－Ｃ，巳分别进行单独培养（单培）或双池培养（双培），内地为Ｇ－Ｃ，外池为Ｔ－Ｃ。４８小时后用放射免疫法（ＲＩＡ）测定培养液中雌二醇（Ｅ２）、睾酮（Ｔ）及孕酮（Ｐ）含量。结果显示：（１）在单培时Ｇ－Ｃ产生极少量Ｅ２，当其与Ｔ－Ｃ双培时则分泌大量Ｅ２；Ｐ和Ｔ产量在双培时比单培时均有增加。（２）Ｔ－Ｃ在单培时产生大量Ｔ，当其与Ｇ－Ｃ双培时，在培液中可测出少量Ｔ；Ｐ和Ｅ２产量在双培时比在单培时均有明显下降。结果表明：在甾体激素生成过程中，Ｇ－Ｃ和Ｔ－Ｃ之间呈现一种旁分泌的调节作用。
Dual cell culture system was used to study the paracrine regulation of G-C and T-C in steroidogenesis. In the experiment, g-C and T-C were isolated from gonads of gilts and cultured separately (single culture) or double-culture (double culture) respectively. The inland area was G-C and the outer pool was T-C. After 48 hours, the contents of estradiol (E2), testosterone (T) and progesterone (P) were determined by radioimmunoassay (RIA). The results showed that: (1) G-C produced a very small amount of E2 in single culture and a large amount of E2 when cultured in double culture with T-C; P and T production increased in double culture compared with single culture. (2) When T-C produced a large amount of T in single culture, a small amount of T could be detected in the culture medium when it was double cultured with G-C; the yield of P and E2 was significantly higher in double culture than in single culture decline. The results showed that during the process of steroidogenesis, there is a paracrine regulation between G-C and T-C.