在常规病理诊断工作中,多采用传统的Ziehl-Neelsen染色法对抗酸杆菌进行染色。但由于抗酸杆菌的菌体含有一层蜡质使染液难以进入,致使染色效果不佳[1]。作者对传统的Ziehl-Neelsen染色法作了一些改进,效果良好,现介绍如下。1材料与方法1.1材料收集厦门大学附属第一医院病理科已确诊结核,Ziehl-Neelsen染色法阳性组织。同时取一块正常组织作阴性对照。1.1.1试剂脱蜡液:汽油松节油等量混合液。苯酚碱性 In routine pathological diagnosis, acid-fast bacilli are often stained using the traditional Ziehl-Neelsen staining method. However, due to acid-fast bacilli bacteria contain a layer of wax so that the dye is difficult to enter, resulting in poor staining [1]. The author made some improvements to the traditional Ziehl-Neelsen dyeing method, the effect is good, are described below. 1 Materials and Methods 1.1 Materials Collection First Affiliated Hospital of Xiamen University Pathology has confirmed tuberculosis, Ziehl-Neelsen staining positive tissue. At the same time take a piece of normal tissue as a negative control. 1.1.1 reagent dewaxing solution: gasoline turpentine equal mixture. Phenol Alkaline