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本文以ＬＳＡＢ免疫组化方法，用单克隆抗体ＰＣ１０和ＤＯ－１对３０例喉鳞癌及１０例对照组（声带息肉）的增殖细胞核抗原（ＰＣＮＡ）与ｐ５３蛋白表达进行研究，探讨其生物学意义。结果发现：１）喉鳞癌组织ＰＣＮＡ阳性表达率为１００％（ＰＣＮＡ－ＬＩｓ１６％～９２％），强阳性率为３６７％（１１／３０），对照组６例阴性，４例弱阳性表达（ＰＣＮＡ－ＬＩｓ＜１０％），试验组阳性表达率及表达程度明显高于对照组（Ｐ＜００５）。２）ｐ５３蛋白在喉鳞癌中阳性表达率为６６７％，对照组全部阴性，有显著差异（Ｐ＜００５）。３）在喉鳞癌组织中，ＰＣＮＡ和ｐ５３蛋白表达与临床分型、Ｔ分期及病理分化程度无显著关系（Ｐ＞００５），与淋巴转移及术后生存率关系密切（Ｐ＜００５）。４）ＰＣＮＡ与ｐ５３蛋白表达正相关。提示：１）喉鳞癌的增殖活性显著增强，ｐ５３基因突变与喉鳞癌的发生和发展有关。２）检测ＰＣＮＡ和ｐ５３蛋白有助于喉鳞癌的早期诊断和鉴别诊断，帮助治疗决策及判断预后。３）ｐ５３对ＰＣＮＡ有调节作用，为研究肿瘤的发生机理和寻找防治对策提供了新的理论依据。
Using LSAB immunohistochemistry, monoclonal antibody PC10 and DO-1 were used to study the expression of proliferating cell nuclear antigen (PCNA) and p53 protein in 30 cases of laryngeal squamous cell carcinoma and 10 cases of vocal cord polyps. significance. The results showed that: 1) The positive expression rate of PCNA in laryngeal squamous cell carcinoma was 100% (PCNA-LIs 16%-92%), and the strong positive rate was 36.7% (11/30). The control group was negative in 6 cases and weakly positive in 4 cases. Expression (PCNA-LIs <10%), the positive expression rate and expression level of the experimental group was significantly higher than the control group (P <0. 05). 2) The positive expression rate of p53 protein in laryngeal squamous cell carcinoma was 66.7%. All of the controls were negative, with significant differences (P < 0.05). 3) In laryngeal squamous cell carcinoma, there was no significant relationship between the expression of PCNA and p53 protein and clinical classification, T stage and pathological differentiation (P>005), which was closely related to lymphatic metastasis and postoperative survival (P < 0 05). 4) PCNA is positively correlated with p53 protein expression. Tip: 1) The proliferative activity of laryngeal squamous cell carcinoma is significantly enhanced. The p53 gene mutation is associated with the occurrence and development of laryngeal squamous cell carcinoma. 2) The detection of PCNA and p53 protein contributes to the early diagnosis and differential diagnosis of laryngeal squamous cell carcinoma and helps in the decision making and prognosis determination. 3) p53 has a regulatory effect on PCNA, providing a new theoretical basis for the study of the mechanism of tumor occurrence and the search for countermeasures.